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Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs

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Neuropeptide Techniques

Part of the book series: Neuromethods ((NM,volume 39))

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Abstract

Neuropeptide receptors can be used as molecular targets to deliver chemotherapeutic drugs into cancer cells. The gastrin-releasing peptide (GRP) receptor was used to deliver a camptothecin (CPT)–bombesin (BB) conjugate into the lung cancer cell line NCI-H1299. The CPT–BB conjugate was metabolized by NCI-H1299 intracellular enzymes releasing the cytotoxic CPT. Receptor binding methods and fluorescent methods are presented for studying the internalization of GRP receptors. The MTT, clonogenic, and 3H-thymidine uptake assays are presented for studying the proliferation of NCI-H1299 cells. The results indicate that CPT-BB conjugates are internalized as a result of receptor-mediated endocytosis.

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Author information

Authors and Affiliations

  1. Department of Health and Human Services, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD, USA

    Terry W. Moody

  2. Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA

    Michael Schumann

  3. Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, USA

    Robert T. Jensen

Authors
  1. Terry W. Moody
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  2. Michael Schumann
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  3. Robert T. Jensen
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Editor information

Editors and Affiliations

  1. Sackler School of Medicine, Tel Aviv University, Israel

    Illana Gozes

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© 2008 Humana Press Inc.

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Moody, T., Schumann, M., Jensen, R. (2008). Cancer Cell Receptor Internalization and Proliferation: Effects of Neuropeptide Analogs. In: Gozes, I. (eds) Neuropeptide Techniques. Neuromethods, vol 39. Humana Press. https://doi.org/10.1007/978-1-60327-099-1_9

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  • DOI: https://doi.org/10.1007/978-1-60327-099-1_9

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-468-5

  • Online ISBN: 978-1-60327-099-1

  • eBook Packages: Springer Protocols

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