The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of “co-crystallants,” and capillary crystallization. “Fragment cocktail crystallography” for medical structural genomics is also described.
Keywords
- Visceral Leishmaniasis
- Target Selection
- Potential Drug Target
- Molecular Replacement
- Protein Crystallization
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Acknowledgments
The authors acknowledge the numerous contributions to SGPP by Ken Stuart, Martin Berg, Keith Hodgson, past SGPP members, advisory committee members, collaborators, industrial partners, and the Protein Structure Initiative (PSI) of NIH (grant GM064655 from NIGMS and NIAID).
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Fan, E. et al. (2008). Structural Genomics of Pathogenic Protozoa: an Overview. In: Kobe, B., Guss, M., Huber, T. (eds) Structural Proteomics. Methods in Molecular Biology™, vol 426. Humana Press. https://doi.org/10.1007/978-1-60327-058-8_33
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