To fit the requirements of structural genomics programs, new as well as classical methods have been adapted to automation. This chapter describes the automated procedure developed within the Structural Biology and Genomics Platform, Strasbourg for performing recombinant protein expression screening in Escherichia coli. The procedure consists of parallel competent cells transformation, cell plating, and liquid culture inoculation, implemented for up to 96 samples at a time.
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Acknowledgments
The authors give special thanks to Dr. Rosalind Kim for critical reading of the manuscript and for useful discussions. The authors also thank David Rosé for his input in the initial stages of the protocol development and for conducting the comparison among different procedures to prepare competent cells for chemical transformation. The Structural Biology and Genomics Department is thanked for supplying plasmid templates. This work was supported by funds from RNG through the Genopole program and SPINE EEC QLG2-CT-2002-00988.
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Busso, D., Stierlé, M., Thierry, JC., Moras, D. (2008). Automated Recombinant Protein Expression Screening in Escherichia coli . In: Kobe, B., Guss, M., Huber, T. (eds) Structural Proteomics. Methods in Molecular Biology™, vol 426. Humana Press. https://doi.org/10.1007/978-1-60327-058-8_10
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DOI: https://doi.org/10.1007/978-1-60327-058-8_10
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