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Transgenesis Techniques pp 73–88Cite as

Pronuclear DNA Injection for the Production of Transgenic Rats

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 561))

Summary

In the absence of germ-line-competent ES cells in the rat, pronuclear microinjection remains an essential tool to generate transgenic rat models. However, DNA microinjection procedures first developed for mouse do not provide scientists with satisfying results when applied to rat. Here we describe optimized procedures for rat with a special focus on rat embryo production, in vitro incubation and culture, DNA pronuclear injection, and the transfer of embryos into foster females.

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References

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Acknowledgments

The author would like to thank staff from the embryology and molecular biology department for their technical help in writing the document.

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Authors and Affiliations

  1. genOway SA, 69362, LYON, France

    Jean Cozzi, Valérie Braun, Anne-Catherine Gross, Christel Merrouche & Yacine Cherifi

  2. Institut National de la Santé et de la Recherche Médicale (INSERM), F-44093, U643 Nantes, France

    Ignacio Anegon

Authors
  1. Jean Cozzi
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  2. Ignacio Anegon
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  3. Valérie Braun
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  4. Anne-Catherine Gross
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  5. Christel Merrouche
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  6. Yacine Cherifi
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Corresponding author

Correspondence to Yacine Cherifi .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Cozzi, J., Anegon, I., Braun, V., Gross, AC., Merrouche, C., Cherifi, Y. (2009). Pronuclear DNA Injection for the Production of Transgenic Rats. In: Cartwright, E. (eds) Transgenesis Techniques. Methods in Molecular Biology, vol 561. Humana Press. https://doi.org/10.1007/978-1-60327-019-9_5

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    • DOI: https://doi.org/10.1007/978-1-60327-019-9_5

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    • Publisher Name: Humana Press

    • Print ISBN: 978-1-60327-018-2

    • Online ISBN: 978-1-60327-019-9

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