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Detection of Herpes Simplex Virus Dependent Apoptosis

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Apoptosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 559))

Summary

Subversion of the host response to virus infection is a universal theme of virology and viral immunology. Multiple mechanisms are in place to limit virus spread on behalf of the host, yet through evolution, viruses have adapted to either weaken or eliminate the effects of these host factors. Cell death or apoptosis is one such example of a host response to viral infection. As such, experimental techniques that enable analysis of viruses (and viral genes) involved in triggering, blocking, or perhaps augmenting this process represent important tools for virologists, immunologists, and cell biologists. Presented here are a series of techniques developed in our lab for the analysis of apoptosis that occurs as a consequence of herpes simplex virus type 1 infection.

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Notes

  1. 1.

    Alternatively, if antibodies containing HRP are utilized during the immunoblotting procedure, traditional methods of detection such as ECL may be substituted here.

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Acknowledgments

We wish to thank all of the individuals in our laboratory whose hard work has set the basis for developing this interesting new research project. Individuals who played significant roles in generating the protocols and the methodologies that served as the basis of this review include Martine Aubert, Jennifer O’Toole, Renee Baranin, Lisa Pomeranz, Christine Sanfilippo, Renzo Lambardozzi, Natalie Chirimuuta, Margot Goodkin, Elise Morton, Jamie Yedowitz, Marie Nguyen, Rachel Kraft, Kristen Pena, Elisabeth Gennis, Fatima Manzoor, and Leah Kang. These studies were supported in part by grants from the United States Public Health Service (AI38873 and AI48582 to J.A.B.) and the American Cancer Society (JFRA 634 to J.A.B.). J.A. Blaho thanks the Lucille P. Markey Charitable Trust and the National Foundation for Infectious Diseases for their support. C. Cotter is a predoctoral trainee and was supported in part by a United States Public Health Service Institutional Research Training Award (AI 07647).

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Authors and Affiliations

  1. Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA

    Christopher R. Cotter & John A. Blaho

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  1. Christopher R. Cotter
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  2. John A. Blaho
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Editors and Affiliations

  1. Boston Biomedical Research Institute, Grove Street 64, Watertown, 02472, U.S.A.

    Peter Erhardt

  2. Boston Biomedical Research Institute, Grove Street 64, Watertown, 02472, U.S.A.

    Ambrus Toth

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Cotter, C.R., Blaho, J.A. (2009). Detection of Herpes Simplex Virus Dependent Apoptosis. In: Erhardt, P., Toth, A. (eds) Apoptosis. Methods in Molecular Biology, vol 559. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-017-5_26

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  • DOI: https://doi.org/10.1007/978-1-60327-017-5_26

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-016-8

  • Online ISBN: 978-1-60327-017-5

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