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An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs

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Drosophila

Part of the book series: Methods in Molecular Biology ((MIMB,volume 420))

Abstract

MicroRNAs (miRNAs), small noncoding RNAs that post-transcriptionally regulate gene expression, are one of the most abundant classes of gene regulators. Yet, little is known about the roles that specific miRNAs play in the development of multicellular organisms. Drosophila provides an excellent model system to explore the in vivo activities of particular miRNAs within the context of well-defined gene-expression programs that control the development of a complex organism. This chapter reviews the various approaches currently used to identify Drosophila miRNAs, detect their expression, determine their messenger RNA targets, and study their function.

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Author information

Authors and Affiliations

  1. Department of Genetics, Dartmouth Medical School, Hanover, NH, USA

    Nicholas S. Sokol

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  1. Nicholas S. Sokol
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Editors and Affiliations

  1. MPI für Molekulare Zellbiologie und Genetik, Pfotenhauerstr. 108, 01307, Dresden, Germany

    Christian Dahmann

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© 2008 Humana Press Inc., Totowa, NJ

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Sokol, N.S. (2008). An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs. In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 420. Humana Press. https://doi.org/10.1007/978-1-59745-583-1_20

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  • DOI: https://doi.org/10.1007/978-1-59745-583-1_20

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-817-1

  • Online ISBN: 978-1-59745-583-1

  • eBook Packages: Springer Protocols

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