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Affinity Chromatography pp 71–90Cite as

Purification of Proteins Using Displacement Chromatography

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 421))

Summary

Displacement chromatography has several advantages over the nonlinear elution technique, as well as the linear elution mode, such as the recovery of purified components at high concentrations, less tailing during elution, high throughput and high resolution. Displacer affinity and its utilization are the critical components of displacement chromatography. Particularly, the nonspecific interactions between the displacer and the stationary phase can be exploited to generate high affinity displacers. This chapter will discuss the design and execution of displacer selection and implementation in a separation specifically focusing on its utilization in ion exchange chromatography.

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Author information

Authors and Affiliations

  1. Bioprocess R&D, BioPurification Development, Merck, Rahway, NJ

    Nihal Tugcu

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  1. Nihal Tugcu
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Editor information

Editors and Affiliations

  1. BioMarin Pharmaceutical Inc., CA

    Michael Zachariou (Director Project Management) (Director Project Management)

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Tugcu, N. (2008). Purification of Proteins Using Displacement Chromatography. In: Zachariou, M. (eds) Affinity Chromatography. Methods in Molecular Biology™, vol 421. Humana Press. https://doi.org/10.1007/978-1-59745-582-4_6

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  • DOI: https://doi.org/10.1007/978-1-59745-582-4_6

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-659-7

  • Online ISBN: 978-1-59745-582-4

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