Abstract
Whole-genome radiation hybrid (RH) mapping has proven to be a powerful tool for mapping genes and comparing genome architecture. We describe a protocol for constructing RH panels by rescuing irradiated fibroblast donor cells of any mammalian species by polyethylene glycol fusion to a thymidine kinase-deficient hamster cell line. Characterization and expansion of a panel of 90–100 cell lines can be used to map virtually any PCR-based marker that can be distinguished from the recipient hamster genome. The described procedure has been used successfully to create RH panels from diverse mammalian species such as macaques, elephants, alpacas, and armadillos, and may be applicable to nonmammalian vertebrates as well.
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References
Walter, M., Spillet, D. J., Thomas, P., Weissenbach, J., and Goodfellow, P. N. (1994) A method for constructing radiation hybrid maps of whole genomes. Nature Genet. 7, 22–28.
Deloukas, P. G. D., Schuler, G., Gyapay, E. M., et al. (1998) A physical map of 30,000 human genes. Science 282, 744–746.
Steen, R. G., Kwitek-Black, A. E., Glenn, C., et al. (1999) A high-density integrated genetic linkage and radiation hybrid map of the laboratory rat. Genome Res. 9, AP1–AP8.
Van Etten, W. J., Steen, R. G., Nguyen, H., et al. (1999) Radiation hybrid map of the mouse genome. Nat. Genet. 22, 384–387.
Watanabe, T. K., Bihoreau, M. T., McCarthy, L. C., et al. (1999) A radiation hybrid map of the rat genome containing 5,255 markers. Nat. Genet. 22, 27–36.
Hitte, C., Madeoy, J., Kirkness, E. F., et al. (2005) Facilitating genome navigation: survey sequencing and dense radiation-hybrid gene mapping. Nat. Rev. Genet. 6, 643–648.
Meyers, S. N., Rogatcheva, M. B., Larkin, D. M., et al. (2005) Piggy-BACing the human genome II. A high-resolution, physically anchored, comparative map of the porcine autosomes. Genomics 86, 739–752.
Everts-van der Wind, A., Larkin, D. M., Green, C. A., et al. (2005) A high-resolution whole-genome cattle-human comparative map reveals details of mammalian chromosome evolution. Proc. Natl Acad. Sci. USA 102, 18,526–18,531.
Brinkmeyer-Langford, C., Raudsepp, T., Lee, E. J., et al. (2005) A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals. Mamm. Genome 16, 631–649.
Murphy, W. J., Davis, B., David, V. A., et al. (2006) A 1.5 megabase resolution radiation hybrid map of the cat genome and comparative analysis with the canine and human genomes. Genomics in press.
Larkin, D. M., Everts-van der Wind, A., Rebeiz, M., et al. (2003) A cattle-human comparative map built with cattle BAC-ends and human genome sequence. Genome Res. 13, 1966–1972.
Murphy, W. J., Larkin, D. M., Everts-van der Wind, A., et al. (2005) Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps. Science 309, 613–617.
Murphy, W. J., Menotti-Raymond, M., Lyons, L. A., Thompson, M. A., and O’Brien, S. J. (1999) Development of a feline whole genome radiation hybrid panel and comparative mapping of human chromosome 12 and 22 loci. Genomics 57, 1–8.
Murphy, W. J., Page, J. E., Smith, C. J., Desrosiers, R. C., and O’Brien, S. J. (2001) A radiation hybrid mapping panel for the rhesus macaque. J. Hered. 92, 516–519.
Kwok, C., Korn, R. M., Davis, M. E., et al. (1998) Characterization of whole genome radiation hybrid mapping resources for non-mammalian vertebrates. Nucleic Acids Res. 26, 3562–3566.
Senger, F., Priat, C., Hitte, C., et al. (2006) The first radiation hybrid map of a perch-like fish: the gilthead seabream (Sparus aurata L). Genomics 87, 793–800.
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© 2008 Humana Press Inc., Totowa, NJ
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Page, J.E., Murphy, W.J. (2008). Construction of Radiation Hybrid Panels. In: Murphy, W.J. (eds) Phylogenomics. Methods in Molecular Biology™, vol 422. Humana Press. https://doi.org/10.1007/978-1-59745-581-7_4
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DOI: https://doi.org/10.1007/978-1-59745-581-7_4
Publisher Name: Humana Press
Print ISBN: 978-1-58829-764-8
Online ISBN: 978-1-59745-581-7
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