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Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues

Comparison of Sensitivity and Specificity

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Vascular Biology Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 139))

Summary

Reactive oxygen and nitrogen species are thought to contribute to pathogenesis of many cardiovascular diseases including hypertension, atherosclerosis, restenosis, heart failure, and diabetic vascular complications. Some of these reactive oxygen species also play an important role in vascular signaling. In this chapter, we describe various techniques that we have successfully employed to reliably measure superoxide and hydrogen peroxide. Because reactive oxygen species are capable of rapidly inactivating nitric oxide and because endothelial function characterized by nitric oxide bioavailability is an important indicator of vascular health, we have also included novel techniques capable of directly measuring nitric oxide radical from vascular cells and tissues.

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Acknowledgments

This work is supported by NIH/NHLBI grants HL077440 and HL081571 American Heart Association Scientist Development Grant (no. 0435189N to H.C.), American Diabetes Association Research Award (H.C.), Career Development Award from the Schweppe Foundation (H.C.), and Start-up Fund from the University of Chicago (H.C.).

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Authors and Affiliations

  1. Division of Molecular Medicine, Departments of Anesthesiology & Medicine Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California, Los Angeles, USA

    Hua Cai

  2. Division of Cardiology, Department of Medicine, Emory, University School of Medicine, Atlanta, Georgia, USA

    Sergey Dikalov, Kathy K. Griendling & David G. Harrison

Authors
  1. Hua Cai
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  2. Sergey Dikalov
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  3. Kathy K. Griendling
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  4. David G. Harrison
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Editors and Affiliations

  1. School of Pharmacy, University of Wyoming, Laramie, Wyoming, USA

    Nair Sreejayan  & Jun Ren  & 

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© 2007 Humana Press Inc.

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Cai, H., Dikalov, S., Griendling, K.K., Harrison, D.G. (2007). Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues. In: Sreejayan, N., Ren, J. (eds) Vascular Biology Protocols. Methods in Molecular Medicine™, vol 139. Humana Press. https://doi.org/10.1007/978-1-59745-571-8_20

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  • DOI: https://doi.org/10.1007/978-1-59745-571-8_20

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-574-3

  • Online ISBN: 978-1-59745-571-8

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