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Lentiviral Transduction of Immune Cells

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Innate Immunity

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 415))

Summary

Gene transfer into mammalian cells has been of crucial importance for studies determining the role of specific genes in the differentiation and cell fate of various hematopoietic lineages. Until recently, the majority of these studies were performed in transformed cell lines due to difficulties in achieving levels of transfection of greater than 1–3% in primary hematopoietic cells. Vectors based on retrovirus and lentivirus backbones have revolutionized our ability to transfer genes into primary hematopoietic cells. These vectors have allowed extensive ex vivo and in vivo studies following introduction of a gene of interest and have been used clinically in individuals suffering from cancers, infections, and genetic diseases. Ex vivo lentiviral gene transfer can result in efficient transduction of progenitor cells (>80%) that can then be further differentiated into immune lineage cells including T, B, dendritic, or natural killer cells. Alternatively, differentiated immune cells can themselves be transduced ex vivo with lentiviral vectors. Here, we discuss optimization of technologies for human immunodeficiency virus (HIV)-based gene transfer into murine and human progenitor and immune cell lineages.

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Authors and Affiliations

  1. Institut de Génétique Moléculaire de Montpellier, Montpellier, France

    Louise Swainson, Cedric Mongellaz, Oumeya Adjali, Rita Vicente & Naomi Taylor

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  1. Louise Swainson
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  2. Cedric Mongellaz
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  3. Oumeya Adjali
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  4. Rita Vicente
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  5. Naomi Taylor
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Editors and Affiliations

  1. Centre d’Immunologie de Marseille-Luminy, INSERM, CNRS Université de la Méditerranée, Marseille, France

    Jonathan Ewbank  & Eric Vivier  & 

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© 2008 Humana Press Inc.

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Swainson, L., Mongellaz, C., Adjali, O., Vicente, R., Taylor, N. (2008). Lentiviral Transduction of Immune Cells. In: Ewbank, J., Vivier, E. (eds) Innate Immunity. Methods in Molecular Biology™, vol 415. Humana Press. https://doi.org/10.1007/978-1-59745-570-1_18

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  • DOI: https://doi.org/10.1007/978-1-59745-570-1_18

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-746-4

  • Online ISBN: 978-1-59745-570-1

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