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Production of Plasmid DNA as Pharmaceutical

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Book cover Gene Therapy of Cancer

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 542))

Summary

Developments in gene therapy, cell therapy, and DNA vaccination require a pharmaceutical gene vector that, on one hand, fulfils the properties to express the encoded information—preferably at the right place, time, and level and, on the other hand, is safe and productive under good manufacturing practices (GMP). Here we summarize the features of producing and modifying these nonviral gene vectors and ensuring the required quality to treat cells and humans or animals.

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Acknowledgments

We thank the German Federal Ministry of Education and Research (BMBF) for grants BioChancePLUS (0313749) and Nano-4-Life (13N9063), the research team of PlasmidFactory, Bielefeld, Germany for contributing to the work and the whole manufacturing team of PlasmidFactory for their discussion. We also thank M. Schmeer and our partners within CLINIGEN (FP6) for critical discussion.

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Authors and Affiliations

  1. PlasmidFactory GmbH & Co. KG, Meisenstrasse 96, D-33739, Bielefeld, Germany

    Martin Schleef

Authors
  1. Martin Schleef
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  2. Markus Blaesen
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Correspondence to Martin Schleef .

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Editors and Affiliations

  1. Molecular Medicine (MDC), Max Delbrück Center for, Robert-Rössle-Str. 10, Berlin, 13125, Germany

    Wolfgang Walther

  2. Molecular Medicine (MDC), Max Delbrück Center for, Robert-Rössle-Str. 10, Berlin, 13125, Germany

    Ulrike S. Stein

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Schleef, M., Blaesen, M. (2009). Production of Plasmid DNA as Pharmaceutical. In: Walther, W., Stein, U. (eds) Gene Therapy of Cancer. Methods in Molecular Biology™, vol 542. Humana Press. https://doi.org/10.1007/978-1-59745-561-9_25

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  • DOI: https://doi.org/10.1007/978-1-59745-561-9_25

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-85-5

  • Online ISBN: 978-1-59745-561-9

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