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Viral Applications of Green Fluorescent Protein pp 309–328Cite as

Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 515))

Summary

Poxviruses including canarypox (ALVAC) and vaccinia viruses have, in recent years, received considerable attention as live vectors for the development of vaccines against infectious diseases such as AIDS, malaria, and tuberculosis. However, the cellular targets for viral infection within the human immune system and the consequences of infection for cells involved in the generation of immune responses have not been clearly delineated. Using recombinant enhanced green fluorescence protein (EGFP)-expressing ALVAC and vaccinia viruses, we have focused here on a side-by-side comparison of ALVAC and vaccinia virus tropism for cells from human peripheral blood and bone marrow. Both ALVAC and vaccinia viruses showed a strong bias toward monocyte infection. ALVAC minimally infected CD19+ B cells and was unable to infect ex vivo NK cells and T lymphocytes, whereas vaccinia virus could infect B lymphocytes and NK cell populations. Vaccinia virus was also able to infect T lymphocytes at low but detectable levels which could be enhanced upon their activation. Both ALVAC and vaccinia viruses could infect immature monocyte-derived dendritic cells (MDDCs), but only ALVAC infection induced their subsequent maturation. Infection in human bone marrow cells showed that ALVAC infection was restricted to a myelomonocytoid cell-specific CD33+ cell population, while vaccinia virus showed a strong, but not exclusive, preference for these cells.

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Acknowledgments

This work was carried out with funding from CFAR at University of Washington (AI 27757), R21 from NIAID/NIH (AI073250), Research Facilities Improvement Program Grant Number C06 RR015481-01 from NIH, Hawaii Community Foundation, OHTN, CIHR, Amfar, and Sanofi-UTRP program. Text and figures were modified from Vaccine (2006) 24, 6376–6391 (33)with permission from the Elsevier Corporate.

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Authors and Affiliations

  1. Hawaii AIDS Clinical Research Program, Department of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA

    Qigui Yu & Ningjie Hu

  2. Department of Immunology, University of Toronto, Toronto, ON, Canada

    Mario Ostrowski

Authors
  1. Qigui Yu
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  2. Ningjie Hu
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  3. Mario Ostrowski
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Correspondence to Qigui Yu .

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Editors and Affiliations

  1. Department of Chemistry, 2355 Fairchild Drive, US Airforce Academy, CO, 80840, USA

    Barry W. Hicks

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Yu, Q., Hu, N., Ostrowski, M. (2009). Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells. In: Hicks, B.W. (eds) Viral Applications of Green Fluorescent Protein. Methods in Molecular Biology™, vol 515. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-559-6_22

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  • DOI: https://doi.org/10.1007/978-1-59745-559-6_22

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-87-9

  • Online ISBN: 978-1-59745-559-6

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