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DNA and RNA Profiling in Human Blood pp 73–87Cite as

Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance

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Part of the book series: METHODS IN MOLECULAR BIOLOGY™ ((MIMB,volume 496))

Abstract

Inflammation is becoming increasingly recognized and discussed as an important pathobiochemical origin in many disease entities such as atherosclerosis, cancer, or infections and genetically determined susceptibility to danger signals may influence the development of inflammatory diseases. Members of the ‘toll-like receptor’ (TLR) family are pivotal molecules in the activation of the innate immune system and specifically recognize structurally conserved pathogen-associated molecular patterns. Since their discovery a growing number of single nucleotide polymorphisms (SNPs) have been identified, functionally characterized and in part linked to multiple diseases. Here we report several protocols for PyrosequencingˆledR approaches to genotype functionally relevant SNPs in TLR-genes and further molecules of the innate immune system.

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Author information

Authors and Affiliations

  1. Institute for Clinical Chemistry, University of Heidelberg, Medical Faculty Mannheim, Mannheim, Germany

    Parviz Ahmad-Nejad (MD)

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  1. Parviz Ahmad-Nejad
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Editor information

Editors and Affiliations

  1. Institute of Transfusion Medicine and Immunology, Heidelberg University Medical Faculty of Mannheim, Mannheim, Germany

    Peter Bugert

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Ahmad-Nejad, P. (2009). Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance. In: Bugert, P. (eds) DNA and RNA Profiling in Human Blood. METHODS IN MOLECULAR BIOLOGY™, vol 496. Humana Press. https://doi.org/10.1007/978-1-59745-553-4_7

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  • DOI: https://doi.org/10.1007/978-1-59745-553-4_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-93-0

  • Online ISBN: 978-1-59745-553-4

  • eBook Packages: Springer Protocols

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