Abstract
Platelet transcriptome studies provide a powerful tool in the analysis of disorders affecting the megakaryocytic-platelet lineage. However, individualised platelet gene expression profiling is hampered by the exceptionally low yield of platelet mRNA. The yield of mRNA transcripts that can be obtained from small-scale platelet preparations is generally not sufficient for standard RNA-labeling reactions used in expression profiling. Furthermore, leukocyte contaminants in platelet preparations are a potential source of ‘unwanted’ mRNA since they contain several orders of magnitude more mRNA than platelets. To overcome these limitations a strategy that combines leukocyte filtration and a PCR-based amplification technique (SMART™TM) has been developed and extensively evaluated.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Rox, J.M., Müller, J., Pötzsch, B. (2009). PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples. In: Bugert, P. (eds) DNA and RNA Profiling in Human Blood. METHODS IN MOLECULAR BIOLOGY™, vol 496. Humana Press. https://doi.org/10.1007/978-1-59745-553-4_18
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DOI: https://doi.org/10.1007/978-1-59745-553-4_18
Publisher Name: Humana Press
Print ISBN: 978-1-934115-93-0
Online ISBN: 978-1-59745-553-4
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