Abstract
Alloimmunization to the blood group antibody anti-RhD (anti-D) is the most common cause of hemolytic disease of the fetus and newborn. Knowledge of fetal D type in women with anti-D makes management of the pregnancy much easier and avoids unnecessary procedures in those women with a D-negative fetus. Fetal D typing can be performed by detection of an RHD gene in cell-free DNA in the plasma of D-negative pregnant women. The technology involves real-time quantitative polymerase chain reactions targeting exons 4, 5, and 10 of RHD, with the exons 4 and 10 tests performed as a multiplex. Testing for SRY in multiplex with the RHD exon 5 test provides an internal control for the presence of fetal DNA when the fetus is male. Fetal D typing has become the standard of care in England in pregnant women with a significant level of anti-D.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Finning, K., Martin, P., Daniels, G. (2009). The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping. In: Bugert, P. (eds) DNA and RNA Profiling in Human Blood. METHODS IN MOLECULAR BIOLOGY™, vol 496. Humana Press. https://doi.org/10.1007/978-1-59745-553-4_11
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DOI: https://doi.org/10.1007/978-1-59745-553-4_11
Publisher Name: Humana Press
Print ISBN: 978-1-934115-93-0
Online ISBN: 978-1-59745-553-4
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