Summary
Slice blotting is a technique for recording the spatial distribution of extracellular signaling molecules released from thin slices of living tissue. Slices are positioned on the surface of a membrane that can trap secreted substances diffusing from the tissue. The pattern of membrane-bound antigens is subsequently visualized by immunoblotting.
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References
Kurien, B.T. and Scofield, R.H. (2006) Western blotting. Methods 38, 283–293.
Kurien, B.T. and Scofield, R.H. (2003) Protein blotting: a review. J Immunol Methods 274, 1–15.
Lowe, G. (1999) Slice blotting: a method for detecting the release of immunoreactive substances from living brain tissue. J Neurosci Methods 90, 117–127.
Paratcha, G., Ibanez, C.F., and Ledda, F. (2006) GDNF is a chemoattractant factor for neuronal precursor cells in the rostral migratory stream. Mol Cell Neurosci. 31, 505–514.
Aghajanian, G.K. and Rasmussen, K (1989) Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices. Synapse 3, 331–338.
Ye, J.H., Zhang, J., Xiao, C., Kong, J.Q. (2006) Patch-clamp studies in the CNS illustrate a simple new method for obtaining viable neurons in rat brain slices: glycerol replacement of NaCl protects CNS neurons. J Neurosci Methods 158, 251–259.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Lowe, G. (2009). Slice Blotting. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_9
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DOI: https://doi.org/10.1007/978-1-59745-542-8_9
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-59745-542-8
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