Summary
Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here, we describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
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References
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Acknowledgements
Special thanks for excellent technical support to Martina Franke (Inst. Immunology, Carl-Gustav Carus TU-Dresden, Germany).
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Franke, C., Gräfe, D., Bartsch, H., Bachmann, M. (2009). Use of non-radioactive detection method for North- and South-Western Blot. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_44
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DOI: https://doi.org/10.1007/978-1-59745-542-8_44
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-73-2
Online ISBN: 978-1-59745-542-8
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