Summary
There are a number of techniques in the scientific world that researchers use to detect specific antigens. One such technique that has provided many advantages over typical immunochemical staining is chemiluminescence. The emission of visible radiation by compounds once exposed to sunlight has been known for centuries and currently is the main principle for chemiluminescence. Here, we introduce three different chemiluminescence techniques that are widely used in immunodetection of antigens: (a) whole membrane chemiluminescence detection, (b) strip membrane chemiluminescence detection, and (c) new line blotting chemiluminescence.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Dorri, Y., Khalili, A.W., Scofield, R.H. (2009). Whole and Strip Nitrocellulose Membrane as well as a New-Line-Immunoblotting ofAntigen Using the Chemiluminescence Technique. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_42
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DOI: https://doi.org/10.1007/978-1-59745-542-8_42
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-73-2
Online ISBN: 978-1-59745-542-8
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