Abstract
Many steps of optimization are needed to achieve large-scale fabrication of high-quality DNA microarrays. These steps involve the printing instrument, the probes to be printed, microarray slides, and spotting buffer together with the surrounding environment, such as humidity and temperature. Robust microarray production requires not only appropriate reagents, equipments, and established procedures, but also devoted and experienced personnel. It is a challenging and craftsman like activity, but at the same time highly rewarding in terms of flexibility and cost efficiency. Outlined here is the workflow of a high-throughput microarray production line.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Waldén, A., Nilsson, P. (2009). Fabrication Using Contact Spotter. In: Dufva, M. (eds) DNA Microarrays for Biomedical Research. Methods in Molecular Biology, vol 529. Humana Press. https://doi.org/10.1007/978-1-59745-538-1_7
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DOI: https://doi.org/10.1007/978-1-59745-538-1_7
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Publisher Name: Humana Press
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