Summary
Intact or genetically manipulated mesnechymal stem cells (MSCs) are being considered an important cell source for developing human cell-based therapeutic approaches. For applications in which transient, high-level expression of the transgene is necessary, adenovirus vectors have become increasingly popular gene-transfer vehicles. However, host range and cell-type tropism restrict the use of specific adenovectors, sometimes necessitating the lengthy development of vectors with appropriate cell specificity. Here, we present a versatile and inexpensive porcine MSC transduction procedure that can also be used on other cell types from various species, including human that are otherwise refractory to adenovirus infection.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Bosch, P., Pratt, S. L., and Stice, S. L. (2006) Isolation, characterization, gene modification and nuclear reprogramming of porcine mesenchymal stem cells. Biol Reprod 74, 46–57.
Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., and Marshak, D. R. (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143–7.
Woodbury, D., Schwarz, E. J., Prockop, D. J., and Black, I. B. (2000) Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 61, 364–70.
Nakamizo, A., Marini, F., Amano, T., Khan, A., Studeny, M., Gumin, J., Chen, J., Hentschel, S., Vecil, G., Dembinski, J., Andreeff, M., and Lang, F. F. (2005) Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas. Cancer Res 65, 3307–18.
Studeny, M., Marini, F. C., Champlin, R. E., Zompetta, C., Fidler, I. J., and Andreeff, M. (2002) Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors. Cancer Res 62, 3603–8.
Studeny, M., Marini, F. C., Dembinski, J. L., Zompetta, C., Cabreira-Hansen, M., Bekele, B. N., Champlin, R. E., and Andreeff, M. (2004) Mesenchymal stem cells: potential precursors for tumor stroma and targeted-delivery vehicles for anticancer agents. J Natl Cancer Inst 96: 1593–603.
Amalfitano, A. (2004) Utilization of adenovirus vectors for multiple gene transfer applications. Methods 33, 173–8.
Thoma, S. J., Lamping, C. P., and Ziegler, B. L. (1994) Phenotype analysis of hematopoietic CD34+ cell populations derived from human umbilical cord blood using flow cytometry and cDNA-polymerase chain reaction. Blood 83, 2103–14.
Rebel, V. I., Hartnett, S., Denham, J., Chan, M., Finberg, R., and Sieff, C. A. (2000) Maturation and lineage-specific expression of the coxsackie and adenovirus receptor in hematopoietic cells. Stem Cells 18, 176–82.
Olmsted-Davis, E. A., Gugala, Z., Gannon, F. H., Yotnda, P., McAlhany, R. E., Lindsey, R. W., and Davis, A. R. (2002) Use of a chimeric adenovirus vector enhances BMP2 production and bone formation. Hum Gene Ther 13, 1337–47.
Kawabata, K., Sakurai, F., Koizumi, N., Hayakawa, T., and Mizuguchi, H. (2006) Adenovirus vector-mediated gene transfer into stem cells. Mol Pharm 3, 95–103.
Yotnda, P., Onishi, H., Heslop, H. E., Shayakhmetov, D., Lieber, A., Brenner, M., and Davis, A. (2001) Efficient infection of primitive hematopoietic stem cells by modified adenovirus. Gene Ther 8, 930–7.
Dodds, E., Piper, T. A., Murphy, S. J., and Dickson, G. (1999) Cationic lipids and polymers are able to enhance adenoviral infection of cultured mouse myotubes. J Neurochem 72, 2105–12.
Byk, T., Haddada, H., Vainchenker, W., and Louache, F. (1998) Lipofectamine and related cationic lipids strongly improve adenoviral infection efficiency of primitive human hematopoietic cells. Hum Gene Ther 9, 2493–502.
Bosch, P., Fouletier-Dilling, C., Olmsted-Davis, E. A., Davis, A. R., and Stice, S. L. (2006) Efficient adenoviral-mediated gene delivery into porcine mesenchymal stem cells. Mol Reprod Dev 73: 1393–403.
Fouletier-Dilling, C. M., Bosch, P., Davis, A. R., Shafer, J. A., Stice, S. L., Gugala, Z., Gannon, F. H., and Olmsted-Davis, E. A. (2005) Novel compound enables high-level adenovirus transduction in the absence of an adenovirus-specific receptor. Hum Gene Ther 16, 1287–97.
Olmsted, E. A., Blum, J. S., Rill, D., Yotnda, P., Gugala, Z., Lindsey, R. W., and Davis, A. R. (2001) Adenovirus-mediated BMP2 expression in human bone marrow stromal cells. J Cell Biochem 82, 11–21.
Acknowledgments
We thank Julie Nelson from the Center for Tropical and Emerging Global Diseases, Flow Cytometry Facility at The University of Georgia for her assistance with flow cytometry analysis and funding from ViaGen.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press
About this protocol
Cite this protocol
Bosch, P., Stice, S.L. (2007). Adenoviral Transduction of Mesenchymal Stem Cells. In: Vemuri, M.C. (eds) Stem Cell Assays. Methods in Molecular Biology™, vol 407. Humana Press. https://doi.org/10.1007/978-1-59745-536-7_18
Download citation
DOI: https://doi.org/10.1007/978-1-59745-536-7_18
Publisher Name: Humana Press
Print ISBN: 978-1-58829-744-0
Online ISBN: 978-1-59745-536-7
eBook Packages: Springer Protocols