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Reaping the Benefits of SAGE

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Plant Bioinformatics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 406))

Summary

Serial analysis of gene expression (SAGE) is a powerful technique which yields a digital measure of gene expression through the sequencing of libraries of specific mRNA-derived fragments, namely SAGE tags. This chapter introduces the methods and software tools that are available for researchers to analyze gene expression through SAGE analysis. A detailed examination of SAGE analysis in Arabidopsis thaliana using the publicly available analysis tool, SaskSAGE, is provided. The use of this software allows the user to maximize the information gained from SAGE experiments in a model system with a fully sequenced genome.

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Acknowledgments

The development of the SaskSAGE website was funded in part by the Genome Prairie project ‘Functional Genomics of Abiotic Stress.’ The authors would like to thank Dr Andrew Sharpe (AAFC, Saskatoon Research Centre) for critical reading of the chapter.

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Authors and Affiliations

  1. Agriculture and Agri-Food, Saskatoon Research Centre, Saskatoon, Saskatchewan, Canada

    Stephen J. Robinson, Justin D. Guenther, Christopher T. Lewis, Matthew G. Links & Isobel A.P. Parkin

Authors
  1. Stephen J. Robinson
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  2. Justin D. Guenther
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  3. Christopher T. Lewis
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  4. Matthew G. Links
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  5. Isobel A.P. Parkin
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Editor information

Editors and Affiliations

  1. Primary Industries Research Victoria, Plant Genetics and Genomics,Victorian AgriBiosciences Centre, La Trobe University, Bundoora, Victoria, Australia

    David Edwards

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© 2007 Humana Press Inc.

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Robinson, S.J., Guenther, J.D., Lewis, C.T., Links, M.G., Parkin, I.A. (2007). Reaping the Benefits of SAGE. In: Edwards, D. (eds) Plant Bioinformatics. Methods in Molecular Biology™, vol 406. Humana Press. https://doi.org/10.1007/978-1-59745-535-0_18

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  • DOI: https://doi.org/10.1007/978-1-59745-535-0_18

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-653-5

  • Online ISBN: 978-1-59745-535-0

  • eBook Packages: Springer Protocols

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