Abstract
Interest in crossover interference in yeast has been spurred by the discovery and characterization of mutants that alter it as well as by the development and testing of models to explain it. This chapter describes methods for detecting and for measuring interference, with emphasis on those that exploit the ability to examine all four products of individual acts of meiosis.
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References
Lhuissier, F. G. P., Offenberg, H. H., Wittich, P. E., Vischer, N. O. E. and Heyting, C. (2007) The mismatch repair protein MLH1 marks a subset of strongly interfering crossovers in tomato. The Plant Cell 19, 862–876.
Fung, J. C., Rockmill, B., Odell, M. and Roeder, G. S. (2004) Imposition of crossover interference through the nonrandom distribution of synapsis initiation complexes. Cell 116, 795–802.
Sturtevant, A. H. (1915) The behavior of the chromosomes, as studied through linkage. Zeit. f. ind. Abst. u. Vereb. 13, 234–287.
Muller, H. J. (1916) The mechanism of crossing over. Am. Nat. 50, 194–221 and ff.
Papazian, H. P. (1952) The analysis of tetrad data. Genetics 37, 175–188.
Haldane, J. B. S. (1919) The combination of linkage values and the calculation of distances between loci of linked factors. J. Genet. 8, 299–309.
Foss, E., Lande, R., Stahl, F. W. and Steinberg, C. M. (1993) Chiasma interference as a function of genetic distance. Genetics 133, 681–691. Corigendum: Genetics 134, 997.
Bailey, N. T. J. (1961) Introduction to the Mathematical Theory of Genetic Linkage, Oxford University Press, London
Zhao, H., McPeek, M. S. and Speed, T. P. (1995a) Statistical analysis of chromatid interference. Genetics 139, 1057–1065.
Perkins, D. D. (1949) Biochemical mutants in the smut fungus Ustilago maydis. Genetics 34, 607–626.
Malkova, A., Swanson, J., German, M., McCusker, J. H., Housworth, E. A., Stahl, F. W. and Haber, J. E. (2004) Gene conversion and crossing over along the 405-kb left arm of Saccharomyces cerevisiae chromosome VII. Genetics 168, 49–63.
McPeek, M. S. and Speed, T. P. (1995) Modeling interference in genetic recombination. Genetics 139, 1031–1044.
Zhao, H., Speed, T. P. and McPeek, M. S. (1995b) Statistical analysis of crossover interference using the Chi-Square model. Genetics 139, 1045–1056.
Broman, K. W. and Weber, J. L. (2001) Characterization of human crossover interference. Am. J. Hum. Genet. 66, 1911–1926.
Kleckner, N., Zickler, D., Jones, G. H., Dekker, J., Padmore, R., Henle, J. and Hutchinson, J. (2004) A mechanical basis for chromosome function. Proc. Natl. Acad. Sci. USA 101, 12592–12597.
Hilliker, A. J. and Chovnick, A. (1981) Further observations on intragenic recombination in Drosophila melanogaster. Genet. Res. 38, 281–296.
Hilliker, A. J., Clark, S. H. and Chovnick, A. (1991) The effect of DNA sequence polymorphisms on intragenic recombination in the rosy locus of Drosophila melanogaster. Genetics 129, 779–781.
Fisher, R. A. (1951) A combinatorial formulation of multiple linkage tests. Nature. 167, 520.
Owen, A. R. G. (1949) The theory of genetical recombination. I. Long chromosome arms. Proc. Roy. Soc. B. 136, 67–94.
Payne, L. C. (1956) The theoryof genetical recombination: a general formulation for a certain class of intercept length distributions appropriate to the discussion of multiple linkage. Proc. Roy. Soc. B. 144, 528–544.
Cobbs, G. (1978) Renewal process approach to the theory of genetic linkage: case of no chromatid interference. Genetics 89, 563–581.
Stamm, P. (1979) Interference in genetic crossing over and chromosome mapping. Genetics 92, 573–594.
Mather, K. (1935) Reductional and equational separation of the chromosomes in bivalents and multivalents. J. Genet. 30, 53–78.
Housworth, E. A. and Stahl, F. W. (2003) Crossover interference in humans. Am. J. Hum. Genet. 73, 188–197.
Lam, S. Y., Horn, S. R., Radford, S. J., Housworth, E. A., Stahl, F. W. and Copenhaver, G. P. (2005) Crossover interference on nucleolus organizing region-bearing chromosomes in Arabidopsis. Genetics 170, 807–812.
Getz, T. J., Banse, S. A., Young, L. S., Banse, A. V., Swanson, J, Wang, G. M., Browne, B. L., Foss, H. M. and Stahl, F. W. (2007) Differential mismatch repair of heteroduplexes distinguishes interfering from “non”-interfering crossing over in Saccharomyces cerevisiae. Genetics 178, 1251–1269.
Stahl, F. W. (2008) On the “NPD ratio” as a test for crossover interference. Genetics 179, 701–704.
Copenhaver, G. P., Housworth, E. A. and Stahl, F. W. (2002) Crossover interference in Arabidopsis. Genetics 160, 1631–1639.
Stahl, F. W. and Lande, R. (1995) Estimating interference and linkage map distance from two-factor tetrad data. Genetics 139, 1449–1454.
Börner, G. V., Kleckner, N. and Hunter, N. (2004) Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis. Cell 117, 29–45.
Zalevsky, J., MacQueen, A. J., Duffy, J. B., Kemphues, K. J. and Villeneuve, A. M. (1999) Crossing over during Caenorhabditis elegans meiosis requires a conserved MutS-based pathway that is partially dispensable in budding yeast. Genetics 153, 1271–1283.
Sturtevant, A. H. (1913) The linear arrangement of six sex-linked factors in Drosophila, as shown by their mode of association. J. Exp. Zool. 14, 43–59.
Strickland, W. N. (1958) An analysis of interference in Aspergillus nidulans. Proc. Roy. Soc. Lond. B. 149, 82–101.
Shinohara, M., Sakai, K., Shinohara, A. and Bishop, D. K. (2003) Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway. Genetics 163, 1273–1286.
Acknowledgments
F.W.S. thanks John P. Nolan for drawing his attention to the view that the distribution of intercrossover distances in the Counting Model is aptly referred to as an Erlang distribution. Tom Petes notified us that the Web site “Stahl Lab Online Tools” mindlessly calculated fN exp values for fT obs > 2/3. It no longer does. Jette Foss provided invaluable editing of the manuscript. The Genetics Community is grateful to Richard Lowry, author of the continually improving, user-friendly web site VassarStats. Dan Graham kindly updated Stahl Lab Online Tools to reflect things we learned during the preparation of this Chapter; in doing so he caught some mistakes in our manuscript. The contribution by E.A.H. was supported by an NSF grant (DMS-0306243) to Indiana University.
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Stahl, F.W., Housworth, E.A. (2009). Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae . In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 557. Humana Press. https://doi.org/10.1007/978-1-59745-527-5_4
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DOI: https://doi.org/10.1007/978-1-59745-527-5_4
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