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Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds

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Book cover Meiosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 557))

Abstract

Different sporulation and pre-sporulation regimens were compared for a number of commonly used laboratory strains of S. cerevisiae to define conditions that support high-efficiency sporulation.

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References

  1. Primig, M., Williams, R. M., Winzeler, E. A., Tevzadze, G. G., Conway, A. R., Hwang, S. Y., Davis, R. W., and Esposito, R. E. (2000) The core meiotic transcriptome in budding yeasts. Nat. Genet. 26, 415–423.

    Article  PubMed  CAS  Google Scholar 

  2. Deutschbauer, A. M. and Davis, R. W. (2005) Quantitative trait loci mapped to single-nucleotide resolution in yeast. Nat. Genet. 37, 1333–1340.

    Article  PubMed  CAS  Google Scholar 

  3. Borner, G. V., Kleckner, N., and Hunter, N. (2004) Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis. Cell 117, 29–45.

    Article  PubMed  Google Scholar 

  4. Kupiec, M., Byers, B., Esposito, R. E., and Mitchell, A. P. (1997) Meiosis and sporulation in Saccharomyces cerevisiae, in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, vol. 3 (Pringle, J. R., Broach, J. R., and Jones, E. W., eds.), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp. 889–1036.

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Acknowledgments

This work was performed in the Department of Food Science and Genetics Graduate Group, University of California, Davis, CA.

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Authors and Affiliations

  1. Center for Excellence in Science and Mathematics Education, College of Science and Mathematics, California Polytechnic State University, San Luis Obispo, CA, USA

    Susan L. Elrod

  2. Department of Pulmonary and Critical Care Medicine, The Permanente Medical Group, Sacramento, CA, USA

    Sabrina M. Chen

  3. Department of Genetics, Stanford University, Stanford, CA, USA

    Katja Schwartz

  4. Academic Advising, General College and College of Arts and Sciences, University of North Carolina, Chapel Hill, NC, USA

    Elizabeth O. Shuster

Authors
  1. Susan L. Elrod
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  2. Sabrina M. Chen
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  3. Katja Schwartz
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  4. Elizabeth O. Shuster
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Editor information

Editors and Affiliations

  1. Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, York Ave. 1275, New York, 10021-6007, U.S.A.

    Scott Keeney

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Elrod, S.L., Chen, S.M., Schwartz, K., Shuster, E.O. (2009). Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds. In: Keeney, S. (eds) Meiosis. Methods in Molecular Biology, vol 557. Humana Press. https://doi.org/10.1007/978-1-59745-527-5_2

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  • DOI: https://doi.org/10.1007/978-1-59745-527-5_2

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-66-4

  • Online ISBN: 978-1-59745-527-5

  • eBook Packages: Springer Protocols

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