abstract
In situ hybridization is well suited to obtaining specific topological information on gene transcripts and thereby to relating such observations to a particular function. In spite of the technical and practical difficulties, the application of molecular biological techniques such as in situ hybridization to the cochlea can provide important insights. However, the rarity of gene products (mRNA and proteins) in the cochlea and its fragile structure require the refinement and adaptation of in situ hybridization methods. The present chapter provides a detailed in situ hybridization protocol adapted to frozen tissue sections collected from adult and neonatal stages of the vertebrate cochlea.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Hiel, H. (2009). In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections. In: Sokolowski, B. (eds) Auditory and Vestibular Research. Methods in Molecular Biology™, vol 493. Humana Press. https://doi.org/10.1007/978-1-59745-523-7_3
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DOI: https://doi.org/10.1007/978-1-59745-523-7_3
Publisher Name: Humana Press
Print ISBN: 978-1-934115-62-6
Online ISBN: 978-1-59745-523-7
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