Abstract
Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are the three leading bacteria species associated with otitis media. Defining the molecular epidemiology of bacteria known to cause otitis media is of great importance, in both clinical and research settings. PFGE and MLST provide data for the characterization of isolates’ genetic relatedness, yet they differ in the types of studies for which they are most useful. Consequently, knowledge of both techniques is important for laboratories intending to study the molecular epidemiology of otitis media–associated bacterial pathogens.
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Acknowledgments
This work was supported by funding awarded to MMP by the National Institute of Allergy and Infectious Diseases (R01 AI068043).
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Thomas, J.C., Pettigrew, M.M. (2009). Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens. In: Sokolowski, B. (eds) Auditory and Vestibular Research. Methods in Molecular Biology™, vol 493. Humana Press. https://doi.org/10.1007/978-1-59745-523-7_11
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DOI: https://doi.org/10.1007/978-1-59745-523-7_11
Publisher Name: Humana Press
Print ISBN: 978-1-934115-62-6
Online ISBN: 978-1-59745-523-7
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