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Application of Sonic Hedgehog to the Developing Chick Limb

  • Eva Tiecke
  • Cheryll Tickle
Part of the Methods Inmolecular Biology™ book series (MIMB, volume 397)

Abstract

Here, we describe methods for applying Sonic hedgehog (Shh) to developing chick limbs. The Sonic hedgehog gene is expressed in the polarizing region, a signaling region at the posterior margin of the limb bud and application of Shh-expressing cells or Shh protein to early limb buds mimics polarizing region signaling. The polarizing region (or zone of polarizing activity) is involved in one of the best known cell-cell interactions in vertebrate embryos and is pivotal in controlling digit number and pattern. At later stages of limb development, the application of Shh protein to the regions between digit primordia can induce changes in digit morphogenesis.

Key Words

Sonic hedgehog chick embryo polarizing region limb development digit morphogenesis bead 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Eva Tiecke
    • 1
  • Cheryll Tickle
    • 1
  1. 1.Division of Cell and Developmental Biology, School of Life SciencesUniversity of DundeeDundeeUK

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