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Using Immunoprecipitation to Study Protein–Protein Interactions in the Hedgehog-Signaling Pathway

  • Chao Tong
  • Jin Jiang
Part of the Methods Inmolecular Biology™ book series (MIMB, volume 397)

Abstract

The Hedgehog (Hh)-signaling pathway has been intensively studied in the past decade. Increasing evidence suggests that dynamic formation of protein complexes plays a critical role in the organization and regulation of Hh signaling. Immunoprecipitation (IP) is a powerful tool to study protein-protein interactions and has provided important insights into the regulation of Hh signal transduction. Here, we show how to use IP to study protein-protein interactions in the Drosophila Hh-signaling pathway.

Key Words

Hedgehog-signaling pathway immunoprecipitation epitope tag Protein A Protein G 

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Copyright information

© Humana Press Inc., Totowa, NJ 2007

Authors and Affiliations

  • Chao Tong
    • 1
  • Jin Jiang
    • 1
  1. 1.Center for Developmental Biology, Department of PharmacologyUniversity of Texas Southwestern Medical CenterDallasUSA

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