Abstract
Salmonellae are mammalian pathogens that are transmitted mainly through foodstuffs and their handlers. Rapid detection requires both specificity and sensitivity in samples containing other bacteria. A solution to this problem is the use of the great specificity conferred by bacteriophages. After implanting reporter genes in a phage genome, the reporter gene products can be measured with great sensitivity when a bacterial host is present. Bacteriophage Felix 01 infects almost all Salmonella strains and has been manipulated to contain the lux genes specifying bacterial luciferase, an enzyme that converts chemical energy to visible light. A widely applicable methodology for preventing the escape of such recombinant phage has also been developed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ulitzur, S. and Kuhn, J. (1989) Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically introduced marker. US Patent No. 4,861,709.
Felix, A. and Callow, B. R. (1943) Typing of paratyphoid B bacilli by means of Vi bacteriophage. Br. Med. J. 2, 127–130.
Kuhn, J., Suissa, M., Wyse, J., et al. (2002) Detection of bacteria using foreign DNA: the development of a bacteriophage reagent for Salmonella. Int. J. Food Microbiol. 74, 229–238.
Fey, H., Burgi, E., Margadant, A., and Boller, E. (1978) An economic and rapid diagnostic procedure for the detection of Salmonella/Shigella using the polyvalent Salmonella phage 0–1. Zent. Bakteriol. Mikrobiol. Hyg. Abt. I Orig. A 240, 7–15.
Kallings, L. O. and Lindberg, A. A. (1967) Resistance to Felix 0–1 phage in Salmonella bacteria. Acta Pathol. Microbiol. Scand. 70, 455–460.
Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Sriranganathan, N., Whichard, J. M., Pierson, F. W., Kapur, V., and Weigt, L. A. Bacteriophage Felix 01, complete genome. NCBI accession number: AF320576.
Kuhn, J., Suissa, M., Chiswell, D., et al. (2002) A bacteriophage reagent for Salmonella: molecular studies on Felix 01. Int. J. Food Microbiol. 74, 217–227.
Stewart, G. S. A. B., Lubinsky-Mink, S., Jackson, C. G., Cassel, A., and Kuhn, J. (1986) pHG165: a pBR322 copy number derivative of pUC8 for cloning and expression. Plasmid 15, 172–181.
Ulizur, S. and Kuhn, J. (2000) Construction of lux bacteriophages and the determination of specific bacteria and their antibiotic sensitivities. Methods Enzymol. 305, 543–557.
Kuhn, J. C., Suissa, M., Chiswell, D., Ulitzur, S., Bar-On, T., and Wyse, J. (1998) Bacteriophage Felix 01 and some of its genes. GenBank accession number AF071201.
Meighen, E. A. (1991) Molecular biology of bacterial bioluminescence. Microbiol. Rev. 55, 123–142.
Kallings, L. O. (1967) Sensitivity of various salmonella strains to Felix 0–1 phage. Acta Pathol. Microbiol. Scand. 70, 446–454.
Stent, G. S. (1963) Molecular Biology of Bacterial Viruses. W.H. Freeman & Co., San Francisco and London, pp. 89–96.
Wolber, P. K. and Green, R. L. (1990) New rapid method for the detection of Salmonella in foods. Trends Food Sci. Technol. 1, 80–82.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Kuhn, J.C. (2007). Detection of Salmonella by Bacteriophage Felix 01. In: Schatten, H., Eisenstark, A. (eds) Salmonella. Methods in Molecular Biology, vol 394. Humana Press. https://doi.org/10.1007/978-1-59745-512-1_2
Download citation
DOI: https://doi.org/10.1007/978-1-59745-512-1_2
Publisher Name: Humana Press
Print ISBN: 978-1-58829-619-1
Online ISBN: 978-1-59745-512-1
eBook Packages: Springer Protocols