Abstract
Protocols are described for the measurement of radioisotopic cation influx and efflux in bacteria using Salmonella as a model system. Methods are discussed for both the use of primary radioisotopes for measurement, e.g., using 54Mn2+ to measure Mn2+ influx, and the use of surrogate radioisotopes when the primary radioisotope is not available, e.g., use of 63Ni2+ or 57Co2+ in place of 28Mg2+ to measure Mg2+ influx. Both vacuum filtration and centrifugation assays are described. In addition, the use and misuse of chelating agents is discussed.
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© 2007 Humana Press Inc., Totowa, NJ
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Maguire, M.E. (2007). Magnesium, Manganese, and Divalent Cation Transport Assays in Intact Cells. In: Schatten, H., Eisenstark, A. (eds) Salmonella. Methods in Molecular Biology, vol 394. Humana Press. https://doi.org/10.1007/978-1-59745-512-1_14
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DOI: https://doi.org/10.1007/978-1-59745-512-1_14
Publisher Name: Humana Press
Print ISBN: 978-1-58829-619-1
Online ISBN: 978-1-59745-512-1
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