Summary
Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-μm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature embryo-derived callus cultures can be transformed using this protocol. To ensure successful reproduction of this protocol, we provide step-by-step laboratory transformation procedures as well as details on growing and caring for transgenic plants in the greenhouse.
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Acknowledgments
The authors wish to thank current and former maize team and greenhouse colleagues, Tina Paque, Marcy Main, Jennie Lund, Jennifer McMurray, and Lise Marcell for their contributions to the development of this protocol. This research is supported partially by the National Science Foundation (DBI #0110023), the Iowa State University Agricultural Experiment Station, the Office of Biotechnology, the Plant Science Institute, and the Baker Endowment Advisory Council for Excellence in Agronomy at Iowa State University.
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Wang, K., Frame, B. (2009). Biolistic Gun-Mediated Maize Genetic Transformation. In: Scott, M.P. (eds) Transgenic Maize. Methods in Molecular Biology™, vol 526. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-494-0_3
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DOI: https://doi.org/10.1007/978-1-59745-494-0_3
Publisher Name: Humana Press, Totowa, NJ
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