Abstract
Patch clamping facilitates directly controlling the voltage of a cell and thus has been acknowledged to be the gold standard for assessing ion-channel physiology in academic and industrial laboratories. The method was developed 30 years ago by (1976), and its various different configurations with glass electrodes have been successfully applied in many labs worldwide (Hamill et al., 1981). The standard approach of patch clamping is done completely manually and requires expensive equipment, such as microscopes, micromanipulators, antivibration tables, and Faraday cages. Further, manual operation is time-consuming, requires experienced personnel, and accommodates only a low amount of throughput per day.
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References
Asmild, M., Oswald, N., Krzywkowski, K. M., et al. (2003) Upscaling and automation of electrophysiology: toward high throughput screening in ion channel drug discovery. Receptors and Channels 9(1), 49–58.
Fejtl, M., Czubayko, U., Hümmer, A., Krauter, T. and Lepple-Wienhues, A. (2005) Automating true manual patch-clamping. Genet. Eng. News 25(14), 37–43.
Fejtl, M., Czubayko, U., Hümmer, A., Krauter, T., and Lepple-Wienhues, A. (2006) Flip-the-Tip: automated patch clamping based on glass electrodes, in Patch Clamp Methods and Protocols (Molnar, P. and Hickman, J. J., eds,), Humana Press, Totowa, NJ.
Goodman, M. B., and Lockery, S. R. (2000) Pressure polishing: a method for reshaping patch pipettes during fire polishing. J. Neurosci. Methods 100(1–2), 13–15.
Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391(2), 85–100.
Lepple-Wienhues, A., Ferlinz, K., Seeger, A., and Schafer, A. (2003) Flip the tip: an automated, high quality, cost-effective patch clamp screen. Receptors and Channels 9(1), 13–17.
Neher, E. and Sakmann, B. (1976) Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 260(5554), 799–802.
Shieh, C. C. (2004) Automated high-throughput patch-clamp techniques. Drug Discov. Today 9, 551–552.
Wang, X. and Li, M. (2003) Automated electrophysiology: high throughput of art. Assay Drug Dev. Technol. 1, 695–708.
Wood, C., Williams, C., and Waldron, G. J. (2004) Patch clamping by numbers. Drug Discov. Today 9, 434–441.
Vasilyev, D. V., Merrill, T. L., and Bowlby, M. R. (2005) Development of a novel automated ion channel recording method using “inside-out” whole-cell membranes. J. Biomol. Screen 10(8), 806–813.
Vasilyev, D. V., Merrill, T., Iwanow, A., Dunlop, J., and Bowlby, M. (2006) A novel method for patch-clamp automation. Pflugers Arch. 452, 240–247.
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© 2007 Humana Press Inc., Totowa, NJ
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Fejtl, M., Czubayko, U., Hümmer, A., Krauter, T., Lepple-Wienhues, A. (2007). Automated Glass Pipette-Based Patch-Clamp Techniques. In: Walz, W. (eds) Patch-Clamp Analysis. Neuromethods, vol 38. Humana Press. https://doi.org/10.1007/978-1-59745-492-6_15
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DOI: https://doi.org/10.1007/978-1-59745-492-6_15
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