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Micro and Nano Technologies in Bioanalysis pp 53–67Cite as

Microfluidic Chips Designed for Measuring Biomolecules Through a Microbead-Based Quantum Dot Fluorescence Assay

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 544))

Summary

This chapter introduces the demonstration of specific antibody detection by using a microbead-based assay with quantum dot (QD) fluorescence on a polydimethylsiloxane (PDMS) microfluidic chip. The microfluidic chip is designed to isolate a single microbead where the binding reaction of antibodies occurs on the surface. The microfluidic chip is fabricated on a glass substrate using a transparent silicone elastomer, PDMS, for easy access of monitoring and flexible gate operations to capture the single microbead. For antibody detection, a sequence of functionalized assays has been performed in the fabricated chip, including the capturing of microbeads, antibody injection into a microchamber, quantum dot injection, and fluorescence detection. Various concentrations of human IgG antibodies have been introduced to bind to a single microbead captured and isolated inside a designated microchamber in a small volume of 75 pL. Fluorescence detection is monitored using a CCD camera after the second binding with the QDs conjugated with anti-human IgG. In this experiment, a human IgG antibody concentration below 0.1 μg/mL has been successfully detected.

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Acknowledgments

The authors acknowledge the financial support from the Intelligent Microsystems Program (IMP) of KIST under the “21C Frontier R&D program” and a research grant from Sogang University in 2007.

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Authors and Affiliations

  1. Department of Electronic Engineering, Sogang University, 1 Shinsu-dong, 121-742, Seoul, Mapo-gu, Korea

    Kwang-Seok Yun

  2. Environment and Energy Division, Korea Institute of Industrial Technology, Cheonan, Korea

    Dohoon Lee

  3. Department of Biological Sciences, KAIST, Daejeon, Korea

    Hak-Sung Kim

  4. Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, USA

    Euisik Yoon

Authors
  1. Kwang-Seok Yun
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  2. Dohoon Lee
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  3. Hak-Sung Kim
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  4. Euisik Yoon
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Correspondence to Kwang-Seok Yun .

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    © 2009 Humana Press, a part of Springer Science+Business Media, LLC

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    Yun, KS., Lee, D., Kim, HS., Yoon, E. (2009). Microfluidic Chips Designed for Measuring Biomolecules Through a Microbead-Based Quantum Dot Fluorescence Assay. In: Foote, R., Lee, J. (eds) Micro and Nano Technologies in Bioanalysis. Methods in Molecular Biology™, vol 544. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-483-4_5

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    • DOI: https://doi.org/10.1007/978-1-59745-483-4_5

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    • Publisher Name: Humana Press, Totowa, NJ

    • Print ISBN: 978-1-934115-40-4

    • Online ISBN: 978-1-59745-483-4

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