Summary
Nonviral gene delivery has been gaining considerable attention recently. Although the efficacy of DNA transfection, which is a major concern, is low in nonviral vector-mediated gene transfer compared with viral ones, nonviral vectors are relatively easy to prepare, less immunogenic and oncogenic, and have no potential of virus recombination and no limitation on the size of a transferred gene. The ability to incorporate genetic materials such as plasmid DNA, RNA, and siRNA into functionalized nanoparticles with little toxicity demonstrates a new era in pharmacotherapy for delivering genes selectively to tissues and cells. In this chapter, we highlight the basic concepts and applications of nonviral gene delivery using super paramagnetic iron oxide nanoparticles and functionalized silica nanoparticles. The experimental protocols related to these topics are described in the chapter.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Tan, W., et al, Bionanotechnology based on silica nanoparticles. Med Res Rev, 2004. 24(5): p. 621–38.
Roy, I., et al, Optical tracking of organically modified silica nanoparticles as DNA carriers: a nonviral, nanomedicine approach for gene delivery. Proc Natl Acad Sci U S A, 2005. 102(2): p. 279–84.
Singh, M., et al, Cationic microparticles: A potent delivery system for DNA vaccines. Proc Natl Acad Sci U S A, 2000. 97(2): p. 811–6.
Bharali, D.J., et al, Organically modified silica nanoparticles: a nonviral vector for in vivo gene delivery and expression in the brain. Proc Natl Acad Sci U S A, 2005. 102(32): p. 11539–44.
Nomura, T., et al, Effect of particle size and charge on the disposition of lipid carriers after intratumoral injection into tissue-isolated tumors. Pharm Res, 1998. 15(1): p. 128–32.
Cuenca, A.G., et al, Emerging implications of nanotechnology on cancer diagnostics and therapeutics. Cancer, 2006. 107(3): p. 459–66.
Kaul, G. and M. Amiji, Cellular interactions and in vitro DNA transfection studies with poly(ethylene glycol)-modified gelatin nanoparticles. J Pharm Sci, 2005. 94(1): p. 184–98.
Kaul, G. and M. Amiji, Tumor-targeted gene delivery using poly(ethylene glycol)-modified gelatin nanoparticles: in vitro and in vivo studies. Pharm Res, 2005. 22(6): p. 951–61.
Kneuer, C., et al, A nonviral DNA delivery system based on surface modified silica-nanoparticles can efficiently transfect cells in vitro. Bioconjug Chem, 2000. 11(6): p. 926–32.
Kneuer, C., et al, Silica nanoparticles modified with aminosilanes as carriers for plasmid DNA. Int J Pharm, 2000. 196(2): p. 257–61.
Mondalek, F.G., et al, The permeability of SPION over an artificial three-layer membrane is enhanced by external magnetic field. J Nanobiotechnology, 2006. 4: p. 4.
Jin, S. and K. Ye, Nanoparticle-mediated drug delivery and gene therapy. Biotechnol Prog, 2007. 23(1): p. 32–41.
Morishita, N., et al, Magnetic nanoparticles with surface modification enhanced gene delivery of HVJ-E vector. Biochem Biophys Res Commun, 2005. 334(4): p. 1121–6.
Prow, T., et al, Construction, gene delivery, and expression of DNA tethered nanoparticles. Mol Vis, 2006. 12: p. 606–15.
Pan, B., et al, Dendrimer-modified magnetic nanoparticles enhance efficiency of gene delivery system. Cancer Res, 2007. 67(17): p. 8156–8163.
Reszka, R., Zhu, J.H., Weber, F., Liposome mediated transfer of marker and cytokine genes into rat and human Glioblastoma cells in vitro and in vivo. J Lipsome Res., 1995. 5: p. 149–154.
Junghans, M., J. Kreuter, and A. Zimmer, Antisense delivery using protamine-oligonucleotide particles. Nucleic Acids Res, 2000. 28(10): p. E45.
Schwab, G., et al, Antisense oligonucleotides adsorbed to polyalkylcyanoacrylate nanoparticles specifically inhibit mutated Ha-ras-mediated cell proliferation and tumorigenicity in nude mice. Proc Natl Acad Sci U S A, 1994. 91(22): p. 10460–4.
Erbacher, P., et al, Chitosan-based vector/DNA complexes for gene delivery: biophysical characteristics and transfection ability. Pharm Res, 1998. 15(9): p. 1332–9.
He, X., Wang, K., Tan, W., Liu, B., Liu, X., Huang, S., Li, D., He, C., Li, J., A novel gene carrier based on amino-modified silica nanoparticles. Chinese Science Bulletin, 2003. 48(3): p. 223–228.
Barnes, A.L., et al, Magnetic characterization of superparamagnetic nanoparticles pulled through model membranes. Biomagn Res Technol, 2007. 5: p. 1.
Fukuda, Y., et al, Superparamagnetic iron oxide (SPIO) MRI contrast agent for bone marrow imaging: differentiating bone metastasis and osteomyelitis. Magn Reson Med Sci, 2006. 5(4): p. 191–6.
Savranoglu, P., et al, The role of SPIO-enhanced MRI in the detection of malignant liver lesions. Clin Imaging, 2006. 30(6): p. 377–81.
Mack, M.G., et al, Superparamagnetic iron oxide-enhanced MR imaging of head and neck lymph nodes. Radiology, 2002. 222(1): p. 239–44.
Luo, D. and W.M. Saltzman, Enhancement of transfection by physical concentration of DNA at the cell surface. Nat Biotechnol, 2000. 18(8): p. 893–5.
Luo, D., et al, A self-assembled, modular DNA delivery system mediated by silica nanoparticles. J Control Release, 2004. 95(2): p. 333–41.
Roy, I., et al, Ceramic-based nanoparticles entrapping water-insoluble photosensitizing anticancer drugs: a novel drug-carrier system for photodynamic therapy. J Am Chem Soc, 2003. 125(26): p. 7860–5.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Jin, S., Leach, J.C., Ye, K. (2009). Nanoparticle-Mediated Gene Delivery. In: Foote, R., Lee, J. (eds) Micro and Nano Technologies in Bioanalysis. Methods in Molecular Biology™, vol 544. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-483-4_34
Download citation
DOI: https://doi.org/10.1007/978-1-59745-483-4_34
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-40-4
Online ISBN: 978-1-59745-483-4
eBook Packages: Springer Protocols