Summary
In this chapter, we describe Raman microspectrophotometry applied to crystals of biomolecules. Raman spectra collected in crystallo provide structural information highly complementary to X-ray diffraction, relate the crystalline state to the solution state, and allow the identification of ligand-bound or intermediate states of macromolecules. Nonresonant Raman spectroscopy is particularly suitable to the study of macromolecular crystals, and therefore applies to a wide range of noncolored crystalline proteins. Practical issues related to the investigation of crystals by Raman microspectrophotometry are reviewed, and the current limitations are highlighted.
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Acknowledgments
This work received financial support from the European MoleÂcular Biology Organisation (EMBO), the European Synchrotron Radiation Facility (Grenoble, France), “Ministère de l’Enseignement et de la Recherche,” and the “RĂ©gion RhĂ´nes-Alpes” (France, CPER and CIBLE contracts). Contributions by Antoine Royant, Vincent Nivière, Jeremy Ohana, David Annequin, and Michel Belleil are acknowledged.
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Bourgeois, D., Katona, G., de Rosny, E., Carpentier, P. (2009). Raman-Assisted X-Ray Crystallography for the Analysis of Biomolecules. In: Foote, R., Lee, J. (eds) Micro and Nano Technologies in Bioanalysis. Methods in Molecular Biology™, vol 544. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-483-4_16
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DOI: https://doi.org/10.1007/978-1-59745-483-4_16
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