Abstract
The plant hormone abscisic acid (ABA) is a major growth regulator mediating several aspects of plant development and stress-induced processes. For the hormone to function within the cell, it must first interact with a proteinaceous receptor to commence transmission of a signal to downstream components that elicit a cellular response. With the identification of several ABA receptors, there is an increased interest in receptor–ABA interaction studies and a demand for easy-to-follow protocols to allow measurement and quantification of ABA binding. Here, we present protocols for radioligand-binding measurements with particular focus on the ABA-binding of the recently identified ABA receptors, ABAP1 and FCA.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Razem, F.A., Hill, R.D. (2009). Binding Assays for Abscisic Acid Receptors. In: Cutler, S., Bonetta, D. (eds) Plant Hormones. Methods in Molecular Biology, vol 495. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-477-3_8
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DOI: https://doi.org/10.1007/978-1-59745-477-3_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-32-9
Online ISBN: 978-1-59745-477-3
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