Abstract
BRI1, a leucine-rich repeat (CRR)-receptor kinase protein, belongs to the plant hormone brassinosteroid (BR) receptor complex in Arabidopsis thaliana (Arabidopsis). The experiments described in this chapter to carry out BR-binding assays in Arabidopsis plants are largely based on previously published procedures by Wang et al. (1) and Caño-Delgado et al. (2), which demonstrated that BRI1 and BRI1-like proteins BRL1 and BRL3, are components of the BR-receptor complexes in vivo. Furthermore, binding assays using a photo-affinity labelled castasterone have demonstrated that BRs are perceived directly by a subdomain of the extracellular domain of BRI1 defined by the 94 amino acids of the ID-LRR22 (3).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Wang, Z. Y., Seto, H., Fujioka, S., Yoshida, S. and Chory, J. (2001) BRI1 is a critical component of a plasma-membrane receptor for plant steroids. Nature 410, 380–383.
Caño-Delgado, A., Yin, Y., Yu, C., Vafeados, D., Mora-García, S., Cheng, J. C., Nam, K. H., Li, J. and Chory, J. (2004) BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis. Development 131, 5341–5351.
Kinoshita, T., Caño-Delgado, A., Seto, H., Hiranuma, S., Fujioka, S., Yoshida, S. and Chory, J. (2005) Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature 433, 167–171.
Clouse, S. D. (2002) Arabidopsis mutants reveal multiple roles for sterols in plant development. Plant Cell 14, 1995–2000.
Belkhadir, Y. and Chory, J. (2006) Brassinosteroid signaling: a paradigm for steroid hormone signaling from the cell surface. Science 314, 1410–1411.
Li, J. and Chory, J. (1997) A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell 90, 929–938.
Friedrichsen, D. M., Joazeiro, C. A., Li, J., Hunter, T. and Chory, J. (2000) Brassinosteroid-insensitive-1 is a ubiquitously expressed leucine-rich repeat receptor serine/threonine kinase. Plant Physiol 123, 1247–1256.
Seto, H.E.A. (1998) A general approach to synthesis of labeled brassinosteroids: preparation of [25,26,27-2H7]brassinolide with 60% isotopic purity from the parent brassinolide. Tetrahedr Lett 39, 7525–7528.
He, Z., Wang, Z. Y., Li, J., Zhu, Q., Lamb, C., Ronald, P. and Chory, J. (2000) Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1. Science 288, 2360–2363.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Caño-Delgado, A., Wang, ZY. (2009). Binding Assays for Brassinosteroid Receptors. In: Cutler, S., Bonetta, D. (eds) Plant Hormones. Methods in Molecular Biology, vol 495. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-477-3_7
Download citation
DOI: https://doi.org/10.1007/978-1-59745-477-3_7
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-32-9
Online ISBN: 978-1-59745-477-3
eBook Packages: Springer Protocols