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Using Reverse Genetics to Develop Small Knockout Collections for Specific Biological Questions

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Book cover Plant Hormones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 495))

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Abstract

With the advent of indexed mutagenized insertion lines in Arabidopsis, it is now possible to order small knockout collections of particular genes to probe a question of biological interest. This first requires querying Arabidopsis databases to identify lines of interest, ordering them and then verifying homozygous lines to make your collection. Once the collection is in hand, it can be used multiple times to test scientific hypotheses as they arise.

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Author information

Authors and Affiliations

  1. Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada

    Julian Northey & Peter McCourt

Authors
  1. Julian Northey
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  2. Peter McCourt
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Editor information

Editors and Affiliations

  1. Dept. Botany, University of Toronto, Willcocks Street 25, Toronto, M5S 3B2, Canada

    Sean Cutler

  2. Dept. Botany, University of Toronto, Willcocks Street 25, Toronto, M5S 3B2, Canada

    Dario Bonetta

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Northey, J., McCourt, P. (2009). Using Reverse Genetics to Develop Small Knockout Collections for Specific Biological Questions. In: Cutler, S., Bonetta, D. (eds) Plant Hormones. Methods in Molecular Biology, vol 495. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-477-3_1

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  • DOI: https://doi.org/10.1007/978-1-59745-477-3_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-32-9

  • Online ISBN: 978-1-59745-477-3

  • eBook Packages: Springer Protocols

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