Abstract
Since the mouse has become the most profound model system to investigate the genetics and pathogenetics of human diseases, a huge number of new mutant mouse strains has been generated and still a lot effort is being done to increase the number of suitable mouse models. In nearly all animal facilities the maintenance of breeding colonies is limited and the mouse strains have to be archived in a reliable way. Mouse sperm cryopreservation provides an efficient management of these genetic resources by reducing maintenance space and cost and by safeguarding them against, for example, disease, breeding failure, and genetic drift.
The sperm archiving method has been proven extensively in large-scale ENU mutagenesis programs and in mouse repository and resource centers worldwide (Federation of International Mouse Resources, http://www.fimre.org). Nevertheless, it is crucial to select accurately the most suitable archiving procedure, or combination of different archiving procedures, for each individual mouse mutant strain.
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References
Tada N, Sato M, Yamanoi J, Mizorogi T, Kasai K, Ogawa S. Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol. J Reprod Fertil 1990; 89(2):511–6.
Yokoyama M, Akiba H, Katsuki M, Nomura T. [Production of normal young following transfer of mouse embryos obtained by in vitro fertilization using cryopreserved spermatozoa]. Jikken Dobutsu 1990; 39(1):125–8.
Marschall S, Huffstadt U, Balling R, Hrabé de Angelis M. Reliable recovery of inbred mouse lines using cryopreserved spermatozoa. Mamm Genome 1999; 10:773–6.
Nakagata N. Use of cryopreservation techniques of embryos and spermatozoa for production of transgenic (Tg) mice and for maintenance of Tg mouse lines. Lab Anim Sci 1996; 46(2):236–8.
Nakagata N. Cryopreservation of mouse spermatozoa. Mamm. Genome 2000; 11(7):572–6.
Thornton CE, Brown SD, Glenister PH. Large numbers of mice established by in vitro fertilization with cryopreserved spermatozoa: implications and applications for genetic resource banks, mutagenesis screens, and mouse backcrosses. Mamm Genome 1999; 10(10):987–92.
Sztein JM, Farley JS, Mobraaten LE. In vitro fertilization with cryopreserved inbred mouse sperm. Biol Reprod 2000; 63(6):1774–80.
Tao J, Du J, Kleinhans FW, Critser ES, Mazur P, Critser JK. The effect of collection temperature, cooling rate and warming rate on chilling injury and cryopreservation of mouse spermatozoa. J Reprod Fertil 1995; 104(2):231–6.
Sztein JM, Farley JS, Young AF, Mobraaten LE. Motility of cryopreserved mouse spermatozoa affected by temperature of collection and rate of thawing. Cryobiology 1997; 35(1):46–52.
Landel CP. Archiving mouse strains by cryopreservation. Lab. Anim. (NY) 2005; 34(4):50–7.
Nishizono H, Shioda M, Takeo T, Irie T, Nakagata N. Decrease of fertilizing ability of mouse spermatozoa after freezing and thawing is related to cellular injury. Biol Reprod 2004; 71(3):973–8.
Hagn M, Marschall S, Hrabe de Angelis MH. EMMA: The European Mouse Mutant Archive. Brief Funct Genomic Proteomic 2007; 6(3):186–92.
Byers SL, Payson SJ, Taft RA. Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology 2006; 65(9):1716–26.
Fuller SJ, Whittingham DG. Capacitation-like changes occur in mouse spermatozoa cooled to low temperatures. Mol Reprod Dev 1997; 46(3):318–24.
Stacy R, Eroglu A, Fowler A, Biggers J, Toner M. Thermal characterization of Nakagata’s mouse sperm freezing protocol. Cryobiology 2006; 52(1):99–107.
Acknowledgments
This work was supported by NGFN2, 01GR0430 and by the European Commission’s FP6 Research Infrastructures Programme. We thank our technicians Steffie Dunst, Monika Beschorner, Alex Huber and Bernhard Rey for their invaluable work.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Marschall, S., Boersma, A., de Angelis, M.H. (2009). Sperm Cryopreservation and In Vitro Fertilization. In: Wurst, W., Kühn, R. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 530. Humana Press. https://doi.org/10.1007/978-1-59745-471-1_22
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DOI: https://doi.org/10.1007/978-1-59745-471-1_22
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