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A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions

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Neutrophil Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 412))

Abstract

Using a combination of induced skin blistering and collection chambers permits dynamic studies of the aseptic inflammatory reaction in humans. Blisters filled with interstitial fluid can be generated by applying negative pressure to normal skin for up to 2 h. The blisters are subsequently denuded to form superficial “skin windows” that are well defined with regard to area and depth. The denuded areas are covered with a separate collection chamber filled with a suitable medium and left for 18–24 h. During this period, neutrophils and inflammatory agents accumulate in the chamber medium, and sequential events in the inflammatory process can be studied by repeated sampling. Inactive medium or isolated peripheral blood cells from the same individual can be used as controls for both cellular functions and the pro-/anti-inflammatory mediators that are generated or released.

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Author information

Authors and Affiliations

  1. Department of Infectious Diseases, University Hospital, Linköping, Sweden

    Per Follin

  2. Department of Rheumatology and Inflammation Research, University of Göteborg, Göteborg, Sweden

    Claes Dahlgren

Authors
  1. Per Follin
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  2. Claes Dahlgren
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Editor information

Editors and Affiliations

  1. Deparment of Veterinary Molecular Biology, Montana State University, Bozeman, MT

    Mark T. Quinn

  2. Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT

    Frank R. DeLeo

  3. Departments of Immunology and Cell Biology, The Scripps Institute, La Jolla, CA

    Gary M. Bokoch

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© 2007 Humana Press Inc., Totowa, NJ

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Follin, P., Dahlgren, C. (2007). A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions. In: Quinn, M.T., DeLeo, F.R., Bokoch, G.M. (eds) Neutrophil Methods and Protocols. Methods in Molecular Biology™, vol 412. Humana Press. https://doi.org/10.1007/978-1-59745-467-4_22

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  • DOI: https://doi.org/10.1007/978-1-59745-467-4_22

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-788-4

  • Online ISBN: 978-1-59745-467-4

  • eBook Packages: Springer Protocols

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