Summary
Targeting recombinant protein production to the periplasmic space of Escherichia coli presents several advantages over cytoplasmic production in inclusion bodies and at the same time overcomes the low productivity problem often associated with culture medium secretion. This chapter presents a strategy for periplasmic production of recombinant proteins fused to synthetic Z domains derived from staphylococcal protein A. Expression, purification, and monitoring strategies are discussed using green fluorescent protein and human proinsulin as model proteins.
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Acknowledgments
Financial support from Fundação para a Ciência e Tecnologia, Minist’erio da Ciência Tecnologia e Ensino Superior, Portugal, is acknowledged.
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Mergulhão, F.J., Monteiro, G.A. (2007). Periplasmic Targeting of Recombinant Proteins in Escherichia coli. In: van der Giezen, M. (eds) Protein Targeting Protocols. Methods in Molecular Biology™, vol 390. Humana Press. https://doi.org/10.1007/978-1-59745-466-7_4
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DOI: https://doi.org/10.1007/978-1-59745-466-7_4
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