Summary
One of the primary goals of genome-wide expression profiling is to identify subsets of gene transcripts that can be associated with specific biological conditions. Once these gene expression “signatures” have been elucidated, they can be used in screening assays to identify compounds that induce similar gene expression changes. To increase the throughput of these screening assays, we have optimized the development of a fluorescent microsphere–based, flow cytometric platform that allows the specific and sensitive screening of up to 100 transcripts per sample in a 96-well plate format. This chapter will outline experimental details of such an experiment using an estrogenic gene expression signature as a specific example.
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References
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Acknowledgments
The authors wish to thank Lynn Jump, Brian Richardson, Cindy Ryan, and Karen Lammers for their assistance in developing this protocol.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Torontali, S.M., Naciff, J.M., Juhlin, K.D., Tiesman, J.P. (2008). Profiling Gene Expression Signatures Using Fluorescent Microspheres. In: Wang, F. (eds) Biomarker Methods in Drug Discovery and Development. Methods in Pharmacology and Toxicology™. Humana Press. https://doi.org/10.1007/978-1-59745-463-6_3
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DOI: https://doi.org/10.1007/978-1-59745-463-6_3
Publisher Name: Humana Press
Print ISBN: 978-1-934115-23-7
Online ISBN: 978-1-59745-463-6
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