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SELDI Technology for Identification of Protein Biomarkers

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Biomarker Methods in Drug Discovery and Development

Part of the book series: Methods in Pharmacology and Toxicology™ ((MIPT))

Summary

The ProteinChip SELDI System and chip arrays provide a sensitive, high-throughput methodology for protein biomarker discovery. The combination of retentate chromatography and time-of-flight mass spectrometry allows for the detection of increased peak numbers even when processing microliter sample volumes. The multiple chip chemistries allow the flexibility for user-defined protocols that can facilitate protein purification strategies. In addition, ProteinChip arrays can be designed to characterize antibody-antigen and other protein-protein interactions. This review will update the reader on the current SELDI methods and protocols that are especially pertinent to protein biomarker discovery.

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Author information

Authors and Affiliations

  1. Nephrology and Hypertension, Cincinnati Children′s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio

    Prasad Devarajan MD

  2. Bio-Rad Laboratories, Hercules, California

    Gary F. Ross PhD

Authors
  1. Prasad Devarajan MD
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  2. Gary F. Ross PhD
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Editor information

Editors and Affiliations

  1. Procter & Gamble Pharmaceutical Inc, Mason, OH, USA, 8700 Mason-Montgomery Road

    Feng Wang PhD

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© 2008 Humana Press, a part of Springer Science+Business Media, LLC

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Devarajan, P., Ross, G.F. (2008). SELDI Technology for Identification of Protein Biomarkers. In: Wang, F. (eds) Biomarker Methods in Drug Discovery and Development. Methods in Pharmacology and Toxicology™. Humana Press. https://doi.org/10.1007/978-1-59745-463-6_12

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  • DOI: https://doi.org/10.1007/978-1-59745-463-6_12

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-934115-23-7

  • Online ISBN: 978-1-59745-463-6

  • eBook Packages: Springer Protocols

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