Summary
Combining serial analysis of gene expression (SAGE) with pyrophosphatase-based ultra-high-throughput DNA sequencing provides increased sensitivity and cost-effective gene expression profiling. The combined techniques obviate the formation and cloning of concatemers and the tedious picking and preparation of sequence templates from bacterial clones that are necessary with SAGE alone. Furthermore, multiplexing of samples or replicates of analysis is included in the experimental design.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Nielsen, K.L. (2008). DeepSAGE. In: Nielsen, K.L. (eds) Serial Analysis of Gene Expression (SAGE). Methods in Molecular Biology™, vol 387. Humana Press. https://doi.org/10.1007/978-1-59745-454-4_6
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DOI: https://doi.org/10.1007/978-1-59745-454-4_6
Publisher Name: Humana Press
Print ISBN: 978-1-58829-676-4
Online ISBN: 978-1-59745-454-4
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