Summary
We will describe a procedure to map epitopes on a protein against monoclonal IgGs. In this procedure, we amplified and mutagenized the entire or a part of the protein. Then, a DNA region encoding the protein was cut out by a restriction enzyme and ligated into a lambda-gt11 expression vector to construct a library. Thus, the protein is expressed as a fusion protein with β-galactosidase. Protein in plaques obtained by phages derived from the library were tested for cross-reactivities by means of immunoblotting experiments.
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Mikawa, T., lkeda, M., Shibata, T. (2009). Epitope Mapping by Region-Specified PCR-Mutagenesis. In: Schutkowski, M., Reineke, U. (eds) Epitope Mapping Protocols. Methods in Molecular Biology™, vol 524. Humana Press. https://doi.org/10.1007/978-1-59745-450-6_22
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DOI: https://doi.org/10.1007/978-1-59745-450-6_22
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