Summary
In vitro culture has been well defined as a useful tool to improve survival and functionality of isolated islets of Langerhans. Evaluation of islet morphology and function is essential prior to use for experimental investigations. Novel techniques such as co-culture and the use of matrices have been shown to improve islet survival and functional viability, and can enhance the purity of the islet preparations, which is particularly important prior to experimental use or patient transplantation.
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Acknowledgements
The author would like to thank Dr. Peter diStefano and Dr. Claire J. Stocker for helpful comments and suggestions.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Nolan, A.L. (2009). In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function. In: Stocker, C. (eds) Type 2 Diabetes. Methods in Molecular Biology, vol 560. Humana Press. https://doi.org/10.1007/978-1-59745-448-3_6
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DOI: https://doi.org/10.1007/978-1-59745-448-3_6
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Publisher Name: Humana Press
Print ISBN: 978-1-934115-15-2
Online ISBN: 978-1-59745-448-3
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