Abstract
Lectins are proteins in nature with molecular weight ranging from 60,000 to 100,000 Da. Many lectins contain covalently bound sugar moieties and are glycoprotein in nature. These are also called phytohemagglutins because they agglutinate red blood cells.
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References
Gordon, J. A., and Marquardt, M. D. (1974) Factors affecting hemagglutination by concanavalin A and soybean agglutinin. Biochim. Biophys. Acta 332, 136–144.
Marquardt, M. D., and Gordon, J. A. (1974) Haemagglutinins in commercial preparations of monsaccharides. Nature 252, 175–176.
Liener, I. E. (1955) Photometric determination of the hemagglutinating activity of soyin and crude soybean extracts. Arch. Biochem. Biophys. 54, 223–231.
Grant, G., More, L. J., McKenzie, N. H., Stewart, J. C., and Pusztai, A. (1983) A survey of the nutritional and haemagglutination properties of legume seeds generally available in the UK. Br. J. Nutr. 50, 207–214.
Lis, H., and Sharon, N. (1972) Soy bean (Glycine max) agglutinin. Methods Enzymol. 28, 360–365.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol Chem. 193, 265–275.
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© 2007 Humana Press Inc., Totowa, NJ
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Makkar, H.P.S., Siddhuraju, P., Becker, K. (2007). Phytohemagglutin/Lectin. In: Plant Secondary Metabolites. Methods in Molecular Biology™, vol 393. Humana Press. https://doi.org/10.1007/978-1-59745-425-4_4
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DOI: https://doi.org/10.1007/978-1-59745-425-4_4
Publisher Name: Humana Press
Print ISBN: 978-1-58829-993-2
Online ISBN: 978-1-59745-425-4
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