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The Spot Technique: Synthesis and Screening of Peptide Macroarrays on Cellulose Membranes

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Peptide-Based Drug Design

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 494))

Summary

Peptide arrays are a widely used tool for drug development. For peptide-based drug design it is necessary to screen a large number of peptides. However, there are often difficulties with this approach. Most common peptide synthesis techniques are able to simultaneously synthesize only up to a few hundred single peptides. Spot synthesis is a positionally addressable, multiple synthesis technique offering the possibility of synthesizing and screening up to 10,000 peptides or peptide mixtures on cellulose or other membrane surfaces. In this chapter we present the basic procedures and screening methods related to spot synthesis and outline protocols for easy-to-use detection methods on these peptide arrays.

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Author information

Authors and Affiliations

  1. Peptide Array Facility of the Brain Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Dirk F.H. Winkler

  2. Biomime Solutions Inc., Surrey, British Columbia, Canada

    William D. Campbell

Authors
  1. Dirk F.H. Winkler
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  2. William D. Campbell
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Editors and Affiliations

  1. Center for Biotechnology, Temple University, Philadelphia, PA 19122

    Laszlo Otvos

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Winkler, D.F., Campbell, W.D. (2008). The Spot Technique: Synthesis and Screening of Peptide Macroarrays on Cellulose Membranes. In: Otvos, L. (eds) Peptide-Based Drug Design. Methods In Molecular Biology™, vol 494. Humana Press. https://doi.org/10.1007/978-1-59745-419-3_4

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  • DOI: https://doi.org/10.1007/978-1-59745-419-3_4

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-990-1

  • Online ISBN: 978-1-59745-419-3

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