Summary
The moss Physcomitrella patens is a long-standing model for studying plant development, growth and cell differentiation in particular. Interest in this non-vascular plant arose following the discovery that homologous recombination is an efficient process. P. patens is, therefore, a tool of choice not only to study gene function but also for recombinant protein production. This system has many attributes that are advantageous for molecular farming: protein production in cell suspension, the possibility of generating targeted knockout mutants for glycoengineering and quantitative optimization for protein production. In terms of technical advances, P. patens is one of the most up-to-date plant expression systems and is a promising alternative to animal cell factories for the production of therapeutic proteins with either simple or highly complex structures.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fischer, R., Twyman, R. M., and Schillberg, S. (2003) Production of antibodies in plants and their use for global health. Vaccine 21, 820–825.
Koprivova, A., Stemmer, C., Altmann, F., Hoffmann, A., Kopriva, S., Gorr, G., Reski, R., and Decker, E. L. (2004) Targeted knockouts of Physcomitrella lacking plant-specific immunogenic N-glycans. Plant Bio-technol. J. 2, 517–523.
Schaefer, D., Zryd, J. P., Knight, C. D., and Cove, D. J. (1991) Stable transformation of the moss Physcomitrella patens. Mol. Gen. Genet. 226, 418–424.
Schaefer, D. G., and Zryd, J. P. (1997) Efficient gene targeting in the moss Physcomitrella patens. Plant J. 11, 1195–1206.
Cove, D. (2005) The moss Physcomitrella patens. Annu. Rev. Genet. 39, 339–358.
Vietor, R., Loutelier, B. C., Fitchette, A., Margerie, P., Gonneau, M., Faye, L., and Lerouge, P. (2003) Protein N-glycosylation is similar in the moss Physcomitrella patens and in higher plants. Planta 218, 269–275.
Gomord, V., and Faye, L. (2004) Posttranslational modification of therapeutic proteins in plants. Curr. Opin. Plant Biol. 7, 171–181.
Gomord, V., Chamberlain, P., Jefferis, R., and Faye, L. (2005) Biopharmaceutical production in plants: problems, solutions and opportunities. Trends Biotechnol. 23, 559–565.
Capecchi, M. R. (2005) Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century. Nat. Rev. Genet. 6, 507–512.
Schaefer, D. G. (2001) Gene targeting in Physcomitrella patens. Curr. Opin. Plant Biol. 4, 143–150.
Schaefer, D. G., and Zryd, J. P. (2001) The moss Physcomitrella patens, now and then. Plant Physiol. 127, 1430–1438.
Trouiller, B., Schaefer, D. G., Charlot, F., and Nogue, F. (2006) MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens. Nucleic Acids Res. 34, 232–242.
Sauer, B. (1993) Manipulation of transgenes by site-specific recombination: use of Cre recombinase. Methods Enzymol. 225, 890–900.
Weise, A., Altmann, F., Rodriguez-Franco, M., Sjoberg, E., Baumer, W., Launhardt, H., Kietzmann, M., and Gorr, G. (2007) High-level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella delta-fuc-t & delta-xyl-t mutant. Plant Biotechnol. J. 5, 389–401.
Ashton, N. W., Cove, D. J., and Featherstone, D. R. (1979) Isolation and physiological analysis of mutants of the moss, Physcomitrella-patens, which over-produce gametophores. Planta 144, 437–442.
Albert, H., Dale, E. C., Lee, E., and Ow, D. W. (1995) Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome. Plant J. 7, 649–659.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Liénard, D., Nogué, F. (2009). Physcomitrella patens : A Non-Vascular Plant for Recombinant Protein Production. In: Faye, L., Gomord, V. (eds) Recombinant Proteins From Plants. Methods in Molecular Biology™, vol 483. Humana Press. https://doi.org/10.1007/978-1-59745-407-0_8
Download citation
DOI: https://doi.org/10.1007/978-1-59745-407-0_8
Publisher Name: Humana Press
Print ISBN: 978-1-58829-978-9
Online ISBN: 978-1-59745-407-0
eBook Packages: Springer Protocols