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Production of Plantibodies in Nicotiana Plants

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Recombinant Proteins From Plants

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 483))

Summary

Because of the wide use and high demand in medicine, monoclonal antibodies are among the main recombinant pharmaceuticals at present, although present limitations of the productive platforms for monoclonal antibodies are driving the improvement of the large-scale technologies and the development of alternative expression systems. This has drawn the attention on plants as expression system for monoclonal antibodies and related derivatives, owning the capacity of plants to properly express and process eukaryotic proteins with biological activity resembling that of the natural proteins. In this chapter, the procedures from the isolation of the monoclonal antibody genes to the biochemical and biological characterization of the plant-expressed monoclonal antibody are described.

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Author information

Authors and Affiliations

  1. Arkansas Biosciences Institute, Arkansas State University, Jonesboro, AR

    Jorge Gavilondo

  2. Center for Genetic Engineering and Biotechnology, Havana, Cuba

    Marta Ayala, Meilyn Rodríguez, Alejandro Fuentes, Gil Enríquez, Lincidio Pérez, José Cremata & Merardo Pujol

Authors
  1. Marta Ayala
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  2. Jorge Gavilondo
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  3. Meilyn Rodríguez
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  4. Alejandro Fuentes
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  5. Gil Enríquez
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  6. Lincidio Pérez
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  7. José Cremata
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  8. Merardo Pujol
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Editor information

Editors and Affiliations

  1. CNRS, Université de Rouen, Mont Saint Aignan, France

    Loïc Faye  & Véronique Gomord  & 

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© 2009 Springer Science+Business Media, LLC

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Ayala, M. et al. (2009). Production of Plantibodies in Nicotiana Plants. In: Faye, L., Gomord, V. (eds) Recombinant Proteins From Plants. Methods in Molecular Biology™, vol 483. Humana Press. https://doi.org/10.1007/978-1-59745-407-0_7

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  • DOI: https://doi.org/10.1007/978-1-59745-407-0_7

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-978-9

  • Online ISBN: 978-1-59745-407-0

  • eBook Packages: Springer Protocols

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