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The Assessment of T-Cell Apoptosis in Synovial Fluid

  • Protocol
Arthritis Research

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 136))

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Abstract

T-cell apoptosis is central to the resolution of chronic inflammation. Inhibition of this process of programmed cell death contributes to disease persistence in conditions such as rheumatoid arthritis. An understanding of T-cell apoptosis and its regulation is clearly important for understanding the pathophysiology of inflammatory disease. This chapter describes a number of apoptosis assays that can be used to measure T-cell apoptosis in synovial fluid. The choice of assay depends, in part, on the phase of apoptosis under investigation and this review puts this into context by introducing these phases and their regulation.

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Author information

Authors and Affiliations

  1. MRC Centre for Immune Regulation, Institute of Biomedical Research Building, University of Birmingham, Birmingham, UK

    Karim Raza, Christopher D. Buckley & Mike Salmon

  2. MRC Centre for Immune Regulation, University of Birmingham, Birmingham, UK

    Dagmar Scheel-Toellner & Janet M. Lord

  3. Department of Immunology and Molecular Pathology, Royal Free and University College Medical School, London, UK

    Arne N. Akbar

Authors
  1. Karim Raza
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  2. Dagmar Scheel-Toellner
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  3. Janet M. Lord
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  4. Arne N. Akbar
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  5. Christopher D. Buckley
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  6. Mike Salmon
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Editor information

Editors and Affiliations

  1. The Kennedy Institute of Rheumatology, Imperial College London, London, UK

    Andrew P. Cope

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© 2007 Humana Press Inc., Totowa, NJ

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Raza, K., Scheel-Toellner, D., Lord, J.M., Akbar, A.N., Buckley, C.D., Salmon, M. (2007). The Assessment of T-Cell Apoptosis in Synovial Fluid. In: Cope, A.P. (eds) Arthritis Research. Methods in Molecular Medicine, vol 136. Humana Press. https://doi.org/10.1007/978-1-59745-402-5_9

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  • DOI: https://doi.org/10.1007/978-1-59745-402-5_9

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-918-5

  • Online ISBN: 978-1-59745-402-5

  • eBook Packages: Springer Protocols

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